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Interestingly, it has recently been shown that regulation of integrin function contributes to the complex behaviors of neural crest cells (Strachan and Condic, 2003, 2004). For example, if a cell expresses both α4β1 and α5β1 integrins, which bind to different regions of FN, expression of ADAM12 could promote use of α5β1 for migration. The ability of ADAM 12 to influence α4β1-mediated cell migration in this defined system was not altered by a point mutation that ablates its proteolytic activity, nor by mutations that prevent removal of its Prodomain, an obligate event for proteolytic activity. Results presented in this study suggest that ADAMs may also influence cell migration in a manner independent of proteolytic activity. For example, ADAM proteases may shed factors that stimulate or direct cell migration (Maheshwari et al., 2001; Mechtersheimer et al., 2001).
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